Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines / (Record no. 3242)

000 -LEADER
fixed length control field 01801nma a2200229 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2013 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 0002-9254
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Cauré Portugal.
245 10 - TITLE STATEMENT
Title Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines /
Statement of responsibility, etc by Cauré Portugal; Fernanda Ruiz-Larrea.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Enology and Viticulture,
Date of publication, distribution, etc 2013.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal American Journal of Enology and Viticulture
520 ## - ABSTRACT
Abstract Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Fernanda Ruiz-Larrea.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME University of La Rioja, Instituto de Ciencias de la Vid y del Vino (CSIC-UR-CAR), Av. Madre de Dios 51, 26006 Logroño, Spain.
773 0# - SOURCE JOURNAL
Journal title American Journal of Enology and Viticulture.
Related parts (Vol.) 64. (No.) 1. 2013. (Pages.) 139-145.
Page numbers 139-145
Volume number 64
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://ajevonline.org/content/64/1/139.abstract
991 ## - OAI IDENTIFIER
OAI set code AJEV
OAI set name American Journal of Enology and Viticulture
100 0# - MAIN ENTRY--PERSONAL NAME
-- 12110
700 0# - ADDED ENTRY-PERSONAL NAME
-- 12111
710 2# - ADDED ENTRY-CORPORATE NAME
-- 12112
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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