The reliability of woody indexing for detection of grapevine virus-associated diseases in three different climatic conditions in Australia (pages 74–80) / (Record no. 3862)

000 -LEADER
fixed length control field 02687nma a2200241 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2013 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1755-0238
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
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-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name F. E. Constable.
245 14 - TITLE STATEMENT
Title The reliability of woody indexing for detection of grapevine virus-associated diseases in three different climatic conditions in Australia (pages 74–80) /
Statement of responsibility, etc by F. E. Constable; J. Connellan; P. Nicholas and B. C. Rodoni.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc Australian Society of Viticulture and Oenology,
Date of publication, distribution, etc 2013.
490 0# - JOURNAL TITLE
Title of the journal Australian Journal of Grape and Wine Research
520 ## - ABSTRACT
Abstract Abstract Background and AimsIn Australia, grapevine varieties are tested for viruses by woody indexing (WI) in combination with other procedures during post-entry quarantine and prior to their introduction into the nucleus collections of high health certification programs. The aim of this study was to determine the reliability and accuracy of WI for virus detection in different climatic conditions. Methods and ResultsReplicated experiments were conducted in a hot climate, a cool climate and a screenhouse. Indicator plants were inoculated with Grapevine virus A, Grapevine virus B, Grapevine fleck virus, Grapevine leafroll-associated virus (GLRaV) 1, GLRaV-2, GLRaV-3, GLRaV-9 and Rupestris stem pitting-associated virus. Indicators were observed during three years for characteristic symptom development and tested for viruses by reverse-transcription polymerase chain reaction. Virus transmission was not always successful. Symptoms were not always observed in each year at each trial site even if viruses could be detected by reverse-transcription polymerase chain reaction. ConclusionsWI reliability was affected by the success of bud-take, transmission of the virus from the candidate bud to the indicator and different climatic conditions. It is recommended that WI be carried out for a minimum of three years in the field. Significance of the StudyUnderstanding the effect of different climatic conditions on WI will improve the reliability of grapevine virus detection in Australia.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME P. Nicholas and B. C. Rodoni.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME J. Connellan.
773 0# - SOURCE JOURNAL
Journal title Australian Journal of Grape and Wine Research
Related parts (Vol.) 19. (No.) issue-1. 2013.
Issue number issue-1
Volume number 19
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URI http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0238.2012.00204.x/references
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0238.2012.00204.x/pdf
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0238.2012.00204.x/full
991 ## - OAI IDENTIFIER
OAI set code AJGWR
OAI set name Australian Journal of Grape and Wine Research
100 0# - MAIN ENTRY--PERSONAL NAME
-- 13538
700 0# - ADDED ENTRY-PERSONAL NAME
-- 13539
700 0# - ADDED ENTRY-PERSONAL NAME
-- 13540
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-- Link to original article.

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