Colonization of Vitis vinifera by a Green Fluorescence Protein-Labeled, gfp-Marked Strain of Xylophilus ampelinus, the Causal Agent of Bacterial Necrosis of Grapevine / (Record no. 45809)

000 -LEADER
fixed length control field 02481nma a2200229 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2003 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Sophie Grall.
245 10 - TITLE STATEMENT
Title Colonization of Vitis vinifera by a Green Fluorescence Protein-Labeled, gfp-Marked Strain of Xylophilus ampelinus, the Causal Agent of Bacterial Necrosis of Grapevine /
Statement of responsibility, etc by Sophie Grall; Charles Manceau.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2003.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract The dynamics of Xylophilus ampelinus were studied in Vitis vinifera cv. Ugni blanc using gfp-marked bacterial strains to evaluate the relative importance of epiphytic and endophytic phases of plant colonization in disease development. Currently, bacterial necrosis of grapevine is of economic importance in vineyards in three regions in France: the Cognac, Armagnac, and Die areas. This disease is responsible for progressive destruction of vine shoots, leading to their death. We constructed gfp-marked strains of the CFBP2098 strain of X. ampelinus for histological studies. We studied the colonization of young plants of V. vinifera cv. Ugni blanc by X. ampelinus after three types of artificial contamination in a growth chamber and in a greenhouse. (i) After wounding of the stem and inoculation, the bacteria progressed down to the crown through the xylem vessels, where they organized into biofilms. (ii) When the bacteria were forced into woody cuttings, they rarely colonized the emerging plantlets. Xylem vessels could play a key role in the multiplication and conservation of the bacteria, rather than being a route for plant colonization. (iii) When bacterial suspensions were sprayed onto the plants, bacteria progressed in two directions: both in emerging organs and down to the crown, thus displaying the importance of epiphytic colonization in disease development.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Charles Manceau.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME UMR Pathologie Végétale, INRA-INH-Université d'Angers, Institut National de la Recherche Agronomique, Centre d'Angers, F-49071 Beaucouzé, France.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 69. (No.) 4. 2003. (Pages.) 1904-1912.
Page numbers 1904-1912
Volume number 69
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/69/4/1904.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331445
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331446
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331447
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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