Karyotype Rearrangements in a Wine Yeast Strain by rad52-Dependent and rad52-Independent Mechanisms / (Record no. 45810)

000 -LEADER
fixed length control field 02416nma a2200253 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2003 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name David Carro.
245 10 - TITLE STATEMENT
Title Karyotype Rearrangements in a Wine Yeast Strain by rad52-Dependent and rad52-Independent Mechanisms /
Statement of responsibility, etc by David Carro; Enric Bartra; Benjamin Piña.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2003.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Yeast strains isolated from the wild may undergo karyotype changes during vegetative growth, a characteristic that compromises their utility in genetic improvement projects for industrial purposes. Karyotype instability is a dominant trait, segregating among meiotic derivatives as if it depended upon only a few genetic elements. We show that disrupting the RAD52 gene in a hypervariable strain partially stabilizes its karyotype. Specifically, RAD52 disruption eliminated recombination at telomeric and subtelomeric sequences, had no influence on ribosomal DNA rearrangement rates, and reduced to 30% the rate of changes in chromosomal size. Thus, there are at least three mechanisms related to karyotype instability in wild yeast strains, two of them not requiring RAD52-mediated homologous recombination. When utilized for a standard sparkling-wine second fermentation, Δrad52 strains retained the enological properties of the parental strain, specifically its vigorous fermentation capability. These data increase our understanding of the mechanisms of karyotype instability in yeast strains isolated from the wild and illustrate the feasibility and limitations of genetic remediation to increase the suitability of natural strains for industrial processes.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Benjamin Piña.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Enric Bartra.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Institut Català de la Vinya i el Vi, 08720 Vilafranca del Penedès, Barcelona, Spain.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 08034 Barcelona.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 69. (No.) 4. 2003. (Pages.) 2161-2165.
Page numbers 2161-2165
Volume number 69
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/69/4/2161.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331848
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331849
700 0# - ADDED ENTRY-PERSONAL NAME
-- 6087
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331450
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331451
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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