Species Differentiation of a Diverse Suite of Bacillus Spores by Mass Spectrometry-Based Protein Profiling / (Record no. 45820)

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fixed length control field 03105nma a2200313 4500
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fixed length control field 2004 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Danielle N. Dickinson.
245 10 - TITLE STATEMENT
Title Species Differentiation of a Diverse Suite of Bacillus Spores by Mass Spectrometry-Based Protein Profiling /
Statement of responsibility, etc by Danielle N. Dickinson.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2004.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract In this study, we demonstrate the versatility of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS) protein profiling for the species differentiation of a diverse suite of Bacillus spores. MALDI-TOFMS protein profiles of 11 different strains of Bacillus spores, encompassing nine different species, were evaluated. Bacillus species selected for MALDI-TOFMS analysis represented the spore-forming bacterial diversity of typical class 100K clean room spacecraft assembly facilities. A one-step sample treatment and MALDI-TOFMS preparation were used to minimize the sample preparation time. A library of MALDI-TOFMS spectra was created from these nine Bacillus species, the most diverse protein profiling study of the genus reported to date. Linear correlation analysis was used to successfully differentiate the MALDI-TOFMS protein profiles from all strains evaluated in this study. The MALDI-TOFMS protein profiles were compared with 16S rDNA sequences for their bacterial systematics and molecular phylogenetic affiliations. The MALDI-TOFMS profiles were found to be complementary to the 16S rDNA analysis. Proteomic studies of Bacillus subtilis 168 were pursued to identify proteins represented by the biomarker peaks in the MALDI-TOFMS spectrum. Four small, acid-soluble proteins (A, B, C, and D), one DNA binding protein, hypothetical protein ymf J, and four proteins associated with the spore coat and spore coat formation (coat JB, coat F, coat T, and spoIVA) were identified. The ability to visualize higher-molecular-mass coat proteins (10 to 25 kDa) as well as smaller proteins (<10 kDa) with MALDI-TOFMS profiling is critical for the complete and effective species differentiation of the Bacillus genus.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Kasthuri Venkateswaran.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Myron T. La Duc.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME David H. Powell.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Igor Gornushkin.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME James D. Winefordner.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME William E. Haskins.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME NASA Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME McKnight Brain Institute, University of Florida, Gainesville, Florida 32611.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Department of Chemistry.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 70. (No.) 1. 2004. (Pages.) 475-482.
Page numbers 475-482
Volume number 70
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URI http://aem.asm.org/content/70/1/475.abstract
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OAI set code AEM
OAI set name Applied and Environmental Microbiology
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-- Link to original article.

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