Molecular and Histological Characterization of Primary (Betaproteobacteria) and Secondary (Gammaproteobacteria) Endosymbionts of Three Mealybug Species / (Record no. 45824)

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fixed length control field 02906nma a2200277 4500
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fixed length control field 2012 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
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-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Laurence N. Gatehouse.
245 10 - TITLE STATEMENT
Title Molecular and Histological Characterization of Primary (Betaproteobacteria) and Secondary (Gammaproteobacteria) Endosymbionts of Three Mealybug Species /
Statement of responsibility, etc by Laurence N. Gatehouse.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2012.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Microscopic localization of endosymbiotic bacteria in three species of mealybug (Pseudococcus longispinus, the long-tailed mealybug; Pseudococcus calceolariae, the citrophilus mealybug; and Pseudococcus viburni, the obscure mealybug) showed these organisms were confined to bacteriocyte cells within a bacteriome centrally located within the hemocoel. Two species of bacteria were present, with the secondary endosymbiont, in all cases, living within the primary endosymbiont. DNA from the dissected bacteriomes of all three species of mealybug was extracted for analysis. Sequence data from selected 16S rRNA genes confirmed identification of the primary endosymbiont as “Candidatus Tremblaya princeps,” a betaproteobacterium, and the secondary endosymbionts as gammaproteobacteria closely related to Sodalis glossinidius. A single 16S rRNA sequence of the primary endosymbiont was found in all individuals of each mealybug species. In contrast, the presence of multiple divergent strains of secondary endosymbionts in each individual mealybug suggests different evolutionary and transmission histories of the two endosymbionts. Mealybugs are known vectors of the plant pathogen Grapevine leafroll-associated virus 3. To examine the possible role of either endosymbiont in virus transmission, an extension of the model for interaction of proteins with bacterial chaperonins, i.e., GroEL protein homologs, based on mobile-loop amino acid sequences of their GroES homologs, was developed and used for analyses of viral coat protein interactions. The data from this model are consistent with a role for the primary endosymbiont in mealybug transmission of Grapevine leafroll-associated virus 3.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Shaun A. Forgie.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME John T. Christeller.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Paul Sutherland.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Ryohei Kaji.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME aPlant and Food Research Institute, Palmerston North, New Zealand.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME bPlant and Food Research Institute, Auckland, New Zealand.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 78. (No.) 4. 2012. (Pages.) 1187-1197.
Page numbers 1187-1197
Volume number 78
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URI http://aem.asm.org/content/78/4/1187.abstract
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OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
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856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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