Metabolic Engineering of the Regulators in Nitrogen Catabolite Repression To Reduce the Production of Ethyl Carbamate in a Model Rice Wine System / (Record no. 45845)

000 -LEADER
fixed length control field 02787nma a2200289 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2014 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Xinrui Zhao.
245 10 - TITLE STATEMENT
Title Metabolic Engineering of the Regulators in Nitrogen Catabolite Repression To Reduce the Production of Ethyl Carbamate in a Model Rice Wine System /
Statement of responsibility, etc by Xinrui Zhao.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2014.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Rice wine has been one of the most popular traditional alcoholic drinks in China. However, the presence of potentially carcinogenic ethyl carbamate (EC) in rice wine has raised a series of food safety issues. During rice wine production, the key reason for EC formation is urea accumulation, which occurs because of nitrogen catabolite repression (NCR) in Saccharomyces cerevisiae. NCR represses urea utilization by retaining Gln3p in the cytoplasm when preferred nitrogen sources are present. In order to increase the nuclear localization of Gln3p, some possible phosphorylation sites on the nuclear localization signal were mutated and the nuclear localization regulation signal was truncated, and the disruption of URE2 provided an additional method of reducing urea accumulation. By combining these strategies, the genes involved in urea utilization (DUR1,2 and DUR3) could be significantly activated in the presence of glutamine. During shake flask fermentations of the genetically modified strains, very little urea accumulated in the medium. Furthermore, the concentrations of urea and EC were reduced by 63% and 72%, respectively, in a model rice wine system. Examination of the normal nutrients in rice wine indicated that there were few differences in fermentation characteristics between the wild-type strain and the genetically modified strain. These results show that metabolic engineering of the NCR regulators has great potential as a method for eliminating EC during rice wine production.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Guocheng Du.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Huijun Zou.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Jian Chen.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Jingwen Zhou.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Jianwei Fu.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME bZhejiang Guyuelongshan Shaoxing Wine Company, Shaoxing, Zhengjiang, China.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME aKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 80. (No.) 1. 2014. (Pages.) 392-398.
Page numbers 392-398
Volume number 80
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/80/1/392.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331588
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-- 331594
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331595
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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