Characterization, Ecological Distribution, and Population Dynamics of Saccharomyces Sensu Stricto Killer Yeasts in the Spontaneous Grape Must Fermentations of Southwestern Spain / (Record no. 45863)

000 -LEADER
fixed length control field 02883nma a2200265 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2012 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Matilde Maqueda.
245 10 - TITLE STATEMENT
Title Characterization, Ecological Distribution, and Population Dynamics of Saccharomyces Sensu Stricto Killer Yeasts in the Spontaneous Grape Must Fermentations of Southwestern Spain /
Statement of responsibility, etc by Matilde Maqueda; Emiliano Zamora; María L. Álvarez; Manuel Ramírez.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2012.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Killer yeasts secrete protein toxins that are lethal to sensitive strains of the same or related yeast species. Among the four types of Saccharomyces killer yeasts already described (K1, K2, K28, and Klus), we found K2 and Klus killer yeasts in spontaneous wine fermentations from southwestern Spain. Both phenotypes were encoded by medium-size double-stranded RNA (dsRNA) viruses, Saccharomyces cerevisiae virus (ScV)-M2 and ScV-Mlus, whose genome sizes ranged from 1.3 to 1.75 kb and from 2.1 to 2.3 kb, respectively. The K2 yeasts were found in all the wine-producing subareas for all the vintages analyzed, while the Klus yeasts were found in the warmer subareas and mostly in the warmer ripening/harvest seasons. The middle-size isotypes of the M2 dsRNA were the most frequent among K2 yeasts, probably because they encoded the most intense K2 killer phenotype. However, the smallest isotype of the Mlus dsRNA was the most frequent for Klus yeasts, although it encoded the least intense Klus killer phenotype. The killer yeasts were present in most (59.5%) spontaneous fermentations. Most were K2, with Klus being the minority. The proportion of killer yeasts increased during fermentation, while the proportion of sensitive yeasts decreased. The fermentation speed, malic acid, and wine organoleptic quality decreased in those fermentations where the killer yeasts replaced at least 15% of a dominant population of sensitive yeasts, while volatile acidity and lactic acid increased, and the amount of bacteria in the tumultuous and the end fermentation stages also increased in an unusual way.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME María L. Álvarez.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Emiliano Zamora.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Manuel Ramírez.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME aDepartamento de Ciencias Biomédicas (Área de Microbiología), Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME bEstación Enológica, Almendralejo, Spain.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 78. (No.) 3. 2012. (Pages.) 735-743.
Page numbers 735-743
Volume number 78
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/78/3/735.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331663
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331664
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331665
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331666
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331667
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331668
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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