Endophytic Colonization of Vitis vinifera L. by Plant Growth-Promoting Bacterium Burkholderia sp. Strain PsJN / (Record no. 45865)

000 -LEADER
fixed length control field 03198nma a2200301 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2005 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Stéphane Compant.
245 10 - TITLE STATEMENT
Title Endophytic Colonization of Vitis vinifera L. by Plant Growth-Promoting Bacterium Burkholderia sp. Strain PsJN /
Statement of responsibility, etc by Stéphane Compant.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2005.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA (PsJN::gusA11) genes were used to enumerate and visualize tissue colonization. The rhizospheres of 4- to 5-week-old plantlets with five developed leaves were inoculated with bacterial suspensions. Epiphytic and endophytic colonization patterns were then monitored by dilution plating assays and microscopic observation of organ sections. Bacteria were chronologically detected first on root surfaces, then in root internal tissues, and finally in the fifth internode and the tissues of the fifth leaf. Analysis of the PsJN colonization patterns showed that this strain colonizes grapevine root surfaces, as well as cell walls and the whole surface of some rhizodermal cells. Cells were also abundant at lateral root emergence sites and root tips. Furthermore, cell wall-degrading endoglucanase and endopolygalacturonase secreted by PsJN explained how the bacterium gains entry into root internal tissues. Host defense reactions were observed in the exodermis and in several cortical cell layers. Bacteria were not observed on stem and leaf surfaces but were found in xylem vessels of the fifth internode and the fifth leaf of plantlets. Moreover, bacteria were more abundant in the fifth leaf than in the fifth internode and were found in substomatal chambers. Thus, it seems that Burkholderia sp. strain PsJN induces a local host defense reaction and systemically spreads to aerial parts through the transpiration stream.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Angela Sessitsch.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Christophe Clément.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Jerzy Nowak.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Birgit Reiter.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Essaïd Ait Barka.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, Reims, France.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Department of Bioresources, ARC Seibersdorf Research GmbH, Seibersdorf, Austria.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 71. (No.) 4. 2005. (Pages.) 1685-1693.
Page numbers 1685-1693
Volume number 71
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URI http://aem.asm.org/content/71/4/1685.abstract
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OAI set code AEM
OAI set name Applied and Environmental Microbiology
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856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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