Deletion of BCY1 from the Saccharomyces cerevisiae Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines / (Record no. 45871)

000 -LEADER
fixed length control field 02140nma a2200241 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2006 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Laura Tabera.
245 10 - TITLE STATEMENT
Title Deletion of BCY1 from the Saccharomyces cerevisiae Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines /
Statement of responsibility, etc by Laura Tabera; Rosario Muñoz; Ramon Gonzalez.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2006.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Autolysis of Saccharomyces cerevisiae is the main source of molecules that contribute to the quality of sparkling wines made by the traditional method. In this work the possibility of accelerating this slow process in order to improve the quality of sparkling wines by using genetically engineered wine yeast strains was explored. The effect of partial or total deletion of BCY1 (which encodes a regulatory subunit of cAMP-dependent protein kinase A) in haploid and diploid (heterozygous and homozygous) yeast strains was studied. We proved that heterozygous strains having partial or complete BCY1 deletions have a semidominant phenotype for several of the properties studied, including autolysis under simulated second-fermentation conditions, in contrast to previously published reports describing mutations in BCY1 as recessive. Considering the degree of autolysis, ethanol tolerance, and technical feasibility, we propose that deletion of the 3′ end of the open reading frame of a single copy of BCY1 is a way to improve the quality of sparkling wines.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Ramon Gonzalez.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Rosario Muñoz.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Department of Microbiology, Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 72. (No.) 4. 2006. (Pages.) 2351-2358.
Page numbers 2351-2358
Volume number 72
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/72/4/2351.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331720
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331721
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331722
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331723
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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