Molecular Detection and Identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in Spoiled Wines / (Record no. 45878)

000 -LEADER
fixed length control field 02443nma a2200265 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2004 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Luca Cocolin.
245 10 - TITLE STATEMENT
Title Molecular Detection and Identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in Spoiled Wines /
Statement of responsibility, etc by Luca Cocolin.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2004.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the DNA from these two species were used. No amplification product was obtained when DNA from other Brettanomyces spp. or wine yeasts were used as the templates. The 305-bp product was subjected to restriction enzyme analysis with DdeI to differentiate between B. bruxellensis and B. anomalus, and each species could be identified on the basis of the different restriction profiles. After optimization of the method by using strains from international collections, wine isolates were tested with the method proposed. Total agreement between traditional identification and molecular identification was observed. The protocol developed was also used for direct detection of B. bruxellensis and B. anomalus in wines suspected to be spoiled by Brettanomyces spp. Application of culture-based and molecular methods led us to the conclusion that 8 of 12 samples were spoiled by B. bruxellensis. Results based on the application of molecular methods suggested that two of the eight positive samples had been infected more recently, since specific signals were obtained at both the DNA and RNA levels.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Lucilla Iacumin.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Giuseppe Comi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Roberto Zironi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Kalliopi Rantsiou.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Dipartimento Scienze degli Alimenti, Università degli studi di Udine, 33100 Udine, Italy.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 70. (No.) 3. 2004. (Pages.) 1347-1355.
Page numbers 1347-1355
Volume number 70
856 42 - ELECTRONIC LOCATION & ACCESS
URI http://aem.asm.org/content/70/3/1347.abstract
991 ## - OAI IDENTIFIER
OAI set code AEM
OAI set name Applied and Environmental Microbiology
100 0# - MAIN ENTRY--PERSONAL NAME
-- 331748
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-- 331749
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331750
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331751
700 0# - ADDED ENTRY-PERSONAL NAME
-- 331752
710 2# - ADDED ENTRY-CORPORATE NAME
-- 331753
856 42 - ELECTRONIC LOCATION & ACCESS
-- Link to original article.

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