A Novel Bacteroidetes Symbiont Is Localized in Scaphoideus titanus, the Insect Vector of Flavescence Dorée in Vitis vinifera / (Record no. 45894)

000 -LEADER
fixed length control field 03700nma a2200445 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 2006 eng
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
ISSN 1098-5336
040 ## - CATALOGING SOURCE
Transcribing agency Sonoma County Wine Library
041 ## -
-- eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Massimo Marzorati.
245 12 - TITLE STATEMENT
Title A Novel Bacteroidetes Symbiont Is Localized in Scaphoideus titanus, the Insect Vector of Flavescence Dorée in Vitis vinifera /
Statement of responsibility, etc by Massimo Marzorati.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc American Society for Microbiology,
Date of publication, distribution, etc 2006.
300 ## - PHYSICAL DESCRIPTION
Type Journal article
490 0# - JOURNAL TITLE
Title of the journal Applied and Environmental Microbiology
520 ## - ABSTRACT
Abstract Flavescence dorée (FD) is a grapevine disease that afflicts several wine production areas in Europe, from Portugal to Serbia. FD is caused by a bacterium, “Candidatus Phytoplasma vitis,” which is spread throughout the vineyards by a leafhopper, Scaphoideus titanus (Cicadellidae). After collection of S. titanus specimens from FD-contaminated vineyards in three different areas in the Piedmont region of Italy, we performed a survey to characterize the bacterial microflora associated with this insect. Using length heterogeneity PCR with universal primers for bacteria we identified a major peak associated with almost all of the individuals examined (both males and females). Characterization by denaturing gradient gel electrophoresis confirmed the presence of a major band that, after sequencing, showed a 97 to 99% identity with Bacteroidetes symbionts of the “Candidatus Cardinium hertigii” group. In addition, electron microscopy of tissues of S. titanus fed for 3 months on phytoplasma-infected grapevine plants showed bacterial cells with the typical morphology of “Ca. Cardinium hertigii.” This endosymbiont, tentatively designated ST1-C, was found in the cytoplasm of previtellogenic and vitellogenic ovarian cells, in the follicle cells, and in the fat body and salivary glands. In addition, cell morphologies resembling those of “Ca. Phytoplasma vitis” were detected in the midgut, and specific PCR assays indicated the presence of the phytoplasma in the gut, fat body and salivary glands. These results indicate that ST1-C and “Ca. Phytoplasma vitis” have a complex life cycle in the body of S. titanus and are colocalized in different organs and tissues.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Fabio Quaglino.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Silvia Corona.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Simona Palermo.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Piero Attilio Bianco.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Tiziana Beninati.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Claudio Bandi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Daniele Daffonchio.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Alberto Alma.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Lorenzo Brusetti.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Rosemarie Tedeschi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Annalisa Balloi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Luciano Sacchi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Massimo Pajoro.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Noura Raddadi.
700 0# - ADDED ENTRY-PERSONAL NAME
PERSONAL NAME Emanuela Clementi.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Dipartimento di Biologia Animale, Università degli Studi di Pavia, 27100 Pavia, Italy.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Dipartimento di Valorizzazione e Protezione delle Risorse Agroforestali, Università degli Studi di Torino, 10095 Grugliasco.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, 20133 Milan.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Istituto di Patologia Vegetale.
710 2# - ADDED ENTRY-CORPORATE NAME
CORPORATE NAME Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche.
773 0# - SOURCE JOURNAL
Journal title Applied and Environmental Microbiology.
Related parts (Vol.) 72. (No.) 2. 2006. (Pages.) 1467-1475.
Page numbers 1467-1475
Volume number 72
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URI http://aem.asm.org/content/72/2/1467.abstract
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OAI set code AEM
OAI set name Applied and Environmental Microbiology
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-- Link to original article.

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