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Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines /

by Cauré Portugal; Fernanda Ruiz-Larrea; University of La Rioja, Instituto de Ciencias de la Vid y del Vino (CSIC-UR-CAR), Av. Madre de Dios 51, 26006 Logroño, Spain.
Material type: materialTypeLabelComputer fileSeries: American Journal of Enology and Viticulture.Publisher: American Society for Enology and Viticulture, 2013Description: Journal article.ISSN: 0002-9254.Online resources: Link to original article. In: American Journal of Enology and Viticulture (Vol.) 64. (No.) 1. 2013. (Pages.) 139-145.Summary: Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.
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Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.

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