The main grapevine germplasm bank of Vitis spp. at the Estación de Viticultura y Enología de Galicia in northwestern Spain includes 272 accessions. Eight SSRs differentiated 66 different genotypes, with 27 groups of synonyms and six homonyms. Brancellao Blanco (white berries) could not be differentiated from Brancellao (black berries). Eleven genotypes had not been previously described. The eight SSRs used in this study confirmed the existence of two groups of cultivars from Galicia: the Caíño group in the west and the Merenzao group in the east.