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Optimization of Sample Preparation and Phloroglucinol Analysis of Marselan Grape Skin Proanthocyanidins using HPLC-DAD-ESI-MS /

by MS.
Material type: materialTypeLabelComputer fileSeries: South African Journal of Enology and Viticulture.Publisher: South African Society for Enology & Viticulture, 2012Description: Journal article.ISSN: 0253-939X.Online resources: Link to original article. | Click here to access online In: South African Journal of Enology and Viticulture Vol. 33. (No.) 1. 2012. p.122-131.Summary: Proanthocyanidins are a group of oligomeric or polymeric flavan-3-ols that are highly significant contributors to astringency in grapes and wines. An orthogonal L9(3)4 test was adopted to determine the optimal extraction conditions and acid-catalysis cleavage of proanthocyanidins in the presence of excess phloroglucinoL The qualitative and quantitative analyses were done using HPLC-DAD-ESI-MSIMS. The results showed that the maximum extraction was obtained using 0.3 moIIL of HCl and 0.005 g of ascorbic acid with incubation at 70°C for 20 mm. The precision and accuracy of this method were acceptable. The composition of free flavan-3-ols and proanthocyanidins in the skins of 'Marselan' grapes (fltis vinifera L. cv.) was investigated. (-)-Epigallocatechin was found to be the most abundant free flavan-3-ol monomer and terminal subunits, whereas the extension subunits were mainly (-)-epicatechin-3-0-gallate in the early developmental stages, and primarily (-)-epigallocatechin and (-)-epicatechin in the middle and late stages.
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Proanthocyanidins are a group of oligomeric or polymeric flavan-3-ols that are highly significant contributors to astringency in grapes and wines. An orthogonal L9(3)4 test was adopted to determine the optimal extraction conditions and acid-catalysis cleavage of proanthocyanidins in the presence of excess phloroglucinoL The qualitative and quantitative analyses were done using HPLC-DAD-ESI-MSIMS. The results showed that the maximum extraction was obtained using 0.3 moIIL of HCl and 0.005 g of ascorbic acid with incubation at 70°C for 20 mm. The precision and accuracy of this method were acceptable. The composition of free flavan-3-ols and proanthocyanidins in the skins of 'Marselan' grapes (fltis vinifera L. cv.) was investigated. (-)-Epigallocatechin was found to be the most abundant free flavan-3-ol monomer and terminal subunits, whereas the extension subunits were mainly (-)-epicatechin-3-0-gallate in the early developmental stages, and primarily (-)-epigallocatechin and (-)-epicatechin in the middle and late stages.

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