000 02748nma a2200361 4500
008 2012 eng
022 _a1098-5336
040 _cscwl
041 _aeng
100 0 _aDavid A. Sela.
_9331669
245 1 0 _aBifidobacterium longum subsp. infantis ATCC 15697 α-Fucosidases Are Active on Fucosylated Human Milk Oligosaccharides /
_cby David A. Sela.
260 _bAmerican Society for Microbiology,
_c2012.
300 _bJournal article
490 0 _aApplied and Environmental Microbiology
520 _aBifidobacterium longum subsp. infantis ATCC 15697 utilizes several small-mass neutral human milk oligosaccharides (HMOs), several of which are fucosylated. Whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed a time-dependent effect. Specifically, among preferred HMOs, tetraose was favored early in fermentation, with other oligosaccharides consumed slightly later. In order to utilize fucosylated oligosaccharides, ATCC 15697 possesses several fucosidases, implicating GH29 and GH95 α-l-fucosidases in a gene cluster dedicated to HMO metabolism. Evaluation of the biochemical kinetics demonstrated that ATCC 15697 expresses three fucosidases with a high turnover rate. Moreover, several ATCC 15697 fucosidases are active on the linkages inherent to the HMO molecule. Finally, the HMO cluster GH29 α-l-fucosidase possesses a crystal structure that is similar to previously characterized fucosidases.
700 0 _aAndrzej Joachimiak.
_9331670
700 0 _aDaniel Garrido.
_9331671
700 0 _aCarlito B. Lebrilla.
_9331672
700 0 _aDavid A. Mills.
_9331673
700 0 _aLarry Lerno.
_9331674
700 0 _aKemin Tan.
_9331675
700 0 _aHyun-Ju Eom.
_9331676
700 0 _aShuai Wu.
_9331677
710 2 _aeRobert Mondavi Institute for Wine and Food Sciences, Department of Viticulture and Enology, University of California, Davis, Davis, California, USA.
_9331678
710 2 _acDepartment of Chemistry, University of California, Davis, Davis, California, USA.
_9331679
710 2 _abFood Science Graduate Group, University of California, Davis, Davis, California, USA.
_9331680
710 2 _adMidwest Center for Structural Genomics and Structural Biology Center, Biosciences, Argonne National Laboratory, Argonne, Illinois, USA.
_9331681
710 2 _aaMicrobiology Graduate Group, University of California, Davis, Davis, California, USA.
_9331682
773 0 _tApplied and Environmental Microbiology.
_g(Vol.) 78. (No.) 3. 2012. (Pages.) 795-803.
_q795-803
_v78
856 4 2 _uhttp://aem.asm.org/content/78/3/795.abstract
_yLink to original article.
942 _cARTICLE
991 _aAEM
_bApplied and Environmental Microbiology
999 _c45864
_d45864